Synaptic plexi of U-fibre layer beneath focal cortical dysplasias: Role in epileptic networks.
Clin Neuropathol
; 37(6): 262-276, 2018.
Article
in En
| MEDLINE
| ID: mdl-30232955
ABSTRACT
AIMS:
The purpose is to demonstrate heterotopic neurones and their synaptic plexi within the U-fibre layer beneath focal cortical dysplasias (FCD). MATERIALS ANDMETHODS:
This prospective qualitative neuropathological study included 23 patients, ages from 3 months to 17 years resections at epileptogenic foci in 10 FCD Ia; 6 FCD IIa,b; 2 FCD IIIa,d; 3 HME; 2 TSC; 8 controls. TECHNIQUES immunoreactivities for synaptophysin, NeuN, MAP2, SMI32, calretinin, GFAP, vimentin, α-B-crystallin. Bielschowsky silver; LFB; mitochondrial enzyme histochemistry (frozen sections).RESULTS:
Subcortical white matter in FCD exhibited neuronal dispersion within U-fibres in FCD I, II and also deep white matter neuronal clusters in FCD II, HME, TSC. Neurones reacted for NeuN, MAP2; few for calretinin. Synaptophysin well demonstrated elaborate U-fibre plexi including axones between U-fibre neurones and projecting to overlying cortex. Deep white matter axones interconnected heterotopia but did not penetrate U-fibres to reach cortex. Mitochondrial enzymatic activity was intense in some neurones, normal in others. Glial α-B-crystallin served as a marker of epileptogenic zones identified electrographically.CONCLUSION:
U-fibre synaptic plexi contribute to excitatory circuitry in the cortex and thus to epileptic networks. Deep white matter neurones form local, less integrated plexi except transmantle dysplasias continuous with cortex. U-fibres may be a barrier to axonal penetration from deep heterotopia. Hypermetabolic neurones suggest repetitive ictogenic depolarizations. Gyral resections should include the U-fibre layer. Neuropathology reports should describe subcortical plexi. Synaptophysin immunoreactivity is a valuable supplement for this purpose.â©.
Full text:
1
Database:
MEDLINE
Main subject:
Axons
/
Brain
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Epilepsy
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Malformations of Cortical Development, Group I
Type of study:
Qualitative_research
Limits:
Adolescent
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Child
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Child, preschool
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Female
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Humans
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Infant
/
Male
Language:
En
Year:
2018
Type:
Article