A novel vertebrate system for the examination and direct comparison of the relative biological effectiveness for different radiation qualities and sources.
Int J Radiat Biol
; 94(11): 985-995, 2018 11.
Article
in En
| MEDLINE
| ID: mdl-30332320
ABSTRACT
PURPOSE:
The recent rapid increase of hadron therapy applications requires the development of high performance, reliable in vivo models for preclinical research on the biological effects of high linear energy transfer (LET) particle radiation.AIM:
The aim of this paper was to test the relative biological effectiveness (RBE) of the zebrafish embryo system at two neutron facilities. MATERIAL ANDMETHODS:
Series of viable zebrafish embryos at 24-hour post-fertilization (hpf) were exposed to single fraction, whole-body, photon and neutron (reactor fission neutrons (RESULTS:
The higher energy of the fast neutron beams represents lower RBE (ref. source LINAC 6 MV photon). The lethality rate in the zebrafish embryo model was 10 times higher for 1 MeV fission neutrons and 2.5 times greater for p (18 MeV)+Be cyclotron generated fast neutron beam when compared to photon irradiation results. Dose-dependent organ perturbations (shortening of the body length, spine curvature, microcephaly, micro-ophthalmia, pericardial edema and inhibition of yolk sac resorption) and microscopic (marked cellular changes in eyes, brain, liver, muscle and the gastrointestinal system) changes scale together with the dose response.CONCLUSION:
The zebrafish embryo system is a powerful and versatile model for assessing the effect of ionizing radiation with different LET values on viability, organ and tissue development.Key words
Full text:
1
Database:
MEDLINE
Main subject:
Zebrafish
/
Linear Energy Transfer
/
Embryo, Nonmammalian
Limits:
Animals
Language:
En
Year:
2018
Type:
Article