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Fluorescence Enhancement of a Microbial Rhodopsin via Electronic Reprogramming.
Marín, María Del Carmen; Agathangelou, Damianos; Orozco-Gonzalez, Yoelvis; Valentini, Alessio; Kato, Yoshitaka; Abe-Yoshizumi, Rei; Kandori, Hideki; Choi, Ahreum; Jung, Kwang-Hwan; Haacke, Stefan; Olivucci, Massimo.
Affiliation
  • Marín MDC; Biotechnology, Pharmacy and Chemistry Department , University of Siena , Siena 53100 , Italy.
  • Agathangelou D; Chemistry Department , Bowling Green State University , Bowling Green , Ohio 43403 , United States.
  • Orozco-Gonzalez Y; University of Strasbourg-CNRS , Institute of Physics and Chemistry of Materials of Strasbourg , 67034 Strasbourg , France.
  • Valentini A; Chemistry Department , Bowling Green State University , Bowling Green , Ohio 43403 , United States.
  • Kato Y; Université de Strasbourg , USIAS Institut d'Études Avanceés , 67083 Strasbourg , France.
  • Abe-Yoshizumi R; Theoretical Physical Chemistry , UR Molsys, University of Liège , Liège , Belgium.
  • Kandori H; Department of Life Science and Applied Chemistry , Nagoya Institute of Technology , Showa-ku , Nagoya 466-8555 , Japan.
  • Choi A; Department of Life Science and Applied Chemistry , Nagoya Institute of Technology , Showa-ku , Nagoya 466-8555 , Japan.
  • Jung KH; OptoBioTechnology Research Center , Nagoya Institute of Technology , Showa-ku , Nagoya 466-8555 , Japan.
  • Haacke S; Department of Life Science and Applied Chemistry , Nagoya Institute of Technology , Showa-ku , Nagoya 466-8555 , Japan.
  • Olivucci M; OptoBioTechnology Research Center , Nagoya Institute of Technology , Showa-ku , Nagoya 466-8555 , Japan.
J Am Chem Soc ; 141(1): 262-271, 2019 01 09.
Article in En | MEDLINE | ID: mdl-30532962
ABSTRACT
The engineering of microbial rhodopsins with enhanced fluorescence is of great importance in the expanding field of optogenetics. Here we report the discovery of two mutants (W76S/Y179F and L83Q) of a sensory rhodopsin from the cyanobacterium Anabaena PCC7120 with opposite fluorescence behavior. In fact, while W76S/Y179F displays, with respect to the wild-type protein, a nearly 10-fold increase in red-light emission, the second is not emissive. Thus, the W76S/Y179F, L83Q pair offers an unprecedented opportunity for the investigation of fluorescence enhancement in microbial rhodopsins, which is pursued by combining transient absorption spectroscopy and multiconfigurational quantum chemistry. The results of such an investigation point to an isomerization-blocking electronic effect as the direct cause of instantaneous (subpicosecond) fluorescence enhancement.
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Full text: 1 Database: MEDLINE Main subject: Protein Engineering / Anabaena / Rhodopsins, Microbial / Mutant Proteins Language: En Year: 2019 Type: Article
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Full text: 1 Database: MEDLINE Main subject: Protein Engineering / Anabaena / Rhodopsins, Microbial / Mutant Proteins Language: En Year: 2019 Type: Article