In Vitro Assay for the Evaluation of Cytotoxic Effects Provided by a Combination of Suicide and Killer Genes in a Bicistronic Vector.

Komissarov, Alexey A; Kostrov, Sergey V; Demidyuk, Ilya V

Komissarov, Alexey A; Kostrov, Sergey V; Demidyuk, Ilya V.

Affiliation

Komissarov AA; Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia.
Kostrov SV; Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia.
Demidyuk IV; Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia. duk@img.ras.ru.

Methods Mol Biol ; 1895: 135-147, 2019.

Article in En | MEDLINE | ID: mdl-30539535

Subject(s)

Cysteine Endopeptidases/genetics; Cytosine Deaminase/genetics; Genes, Transgenic, Suicide; Genetic Therapy/methods; Genetic Vectors; Pentosyltransferases/genetics; Viral Proteins/genetics; 3C Viral Proteases; Fungal Proteins; Humans; Plasmids; Recombinant Fusion Proteins/genetics; Yeasts/enzymology

Key words

Bicistronic expression vector; Bifunctional yeast cytosine deaminase/uracil phosphoribosyltransferase fusion protein FCU1; Colorimetric cell viability assay; Human hepatitis A virus 3C protease; Suicide gene therapy; Transient transfection

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Full text: 1 Database: MEDLINE Main subject: Pentosyltransferases / Viral Proteins / Cysteine Endopeptidases / Genetic Therapy / Cytosine Deaminase / Genes, Transgenic, Suicide / Genetic Vectors Limits: Humans Language: En Year: 2019 Type: Article

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