Structure-guided design combined with evolutionary diversity led to the discovery of the xylose-releasing exo-xylanase activity in the glycoside hydrolase family 43.

Zanphorlin, Letícia Maria; de Morais, Mariana Abrahão Bueno; Diogo, José Alberto; Domingues, Mariane Noronha; de Souza, Flávio Henrique Moreira; Ruller, Roberto; Murakami, Mario Tyago

Zanphorlin, Letícia Maria; de Morais, Mariana Abrahão Bueno; Diogo, José Alberto; Domingues, Mariane Noronha; de Souza, Flávio Henrique Moreira; Ruller, Roberto; Murakami, Mario Tyago.

Affiliation

Zanphorlin LM; Brazilian Bioethanol Science and Technology Laboratory, National Center for Research in Energy and Materials, Campinas, São Paulo, Brazil.
de Morais MAB; Brazilian Bioethanol Science and Technology Laboratory, National Center for Research in Energy and Materials, Campinas, São Paulo, Brazil.
Diogo JA; Brazilian Bioethanol Science and Technology Laboratory, National Center for Research in Energy and Materials, Campinas, São Paulo, Brazil.
Domingues MN; Brazilian Bioethanol Science and Technology Laboratory, National Center for Research in Energy and Materials, Campinas, São Paulo, Brazil.
de Souza FHM; Brazilian Bioethanol Science and Technology Laboratory, National Center for Research in Energy and Materials, Campinas, São Paulo, Brazil.
Ruller R; Brazilian Bioethanol Science and Technology Laboratory, National Center for Research in Energy and Materials, Campinas, São Paulo, Brazil.
Murakami MT; Brazilian Bioethanol Science and Technology Laboratory, National Center for Research in Energy and Materials, Campinas, São Paulo, Brazil.

Biotechnol Bioeng ; 116(4): 734-744, 2019 04.

Article in En | MEDLINE | ID: mdl-30556897

Subject(s)

Bacillus licheniformis/enzymology; Xylose/metabolism; Xylosidases/genetics; Xylosidases/metabolism; Bacillus licheniformis/chemistry; Bacillus licheniformis/genetics; Bacillus licheniformis/metabolism; Catalytic Domain; Crystallography, X-Ray; Enzyme Activation; Hydrogen Bonding; Models, Molecular; Mutagenesis, Site-Directed; Protein Conformation; Protein Multimerization; Substrate Specificity; Xylosidases/chemistry

Key words

4-xylanase; evolutionary diversity; exo-ß-1; glycosidase hydrolase family 43; molecular mechanism; rational redesign

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Full text: 1 Database: MEDLINE Main subject: Xylose / Xylosidases / Bacillus licheniformis Language: En Year: 2019 Type: Article

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Full text: 1 Database: MEDLINE Main subject: Xylose / Xylosidases / Bacillus licheniformis Language: En Year: 2019 Type: Article