Autophagy Pathway Mapping to Elucidate the Function of Novel Autophagy Regulators Identified by High-Throughput Screening.

Wirth, Martina; Tooze, Sharon A

Wirth, Martina; Tooze, Sharon A.

Affiliation

Wirth M; Molecular Cell Biology of Autophagy Laboratory, The Francis Crick Institute, London, UK.
Tooze SA; Molecular Cell Biology of Autophagy Laboratory, The Francis Crick Institute, London, UK. Sharon.tooze@crick.ac.uk.

Methods Mol Biol ; 1880: 375-387, 2019.

Article in En | MEDLINE | ID: mdl-30610711

Subject(s)

Autophagy/physiology; High-Throughput Screening Assays/methods; Protein Interaction Mapping/methods; Signal Transduction/physiology; Autophagosomes/metabolism; Autophagy-Related Proteins/metabolism; HEK293 Cells; High-Throughput Screening Assays/instrumentation; Humans; Intracellular Membranes/metabolism; Lysosomes/metabolism; Membrane Proteins/metabolism; Microscopy, Fluorescence/instrumentation; Microscopy, Fluorescence/methods; Microtubule-Associated Proteins/metabolism; Protein Interaction Mapping/instrumentation

Key words

ATG9; Autophagic flux; Autophagosome maturation; Autophagy pathway mapping; LC3; ULK complex; WIPI2; mTORC1

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Full text: 1 Database: MEDLINE Main subject: Autophagy / Signal Transduction / Protein Interaction Mapping / High-Throughput Screening Assays Type of study: Diagnostic_studies / Screening_studies Limits: Humans Language: En Year: 2019 Type: Article

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