Curcumin improves perfusion recovery in experimental peripheral arterial disease by upregulating microRNA-93 expression.

ABSTRACT

In peripheral arterial disease (PAD), angiogenesis is the major process involved in repairing the microvasculature in the ischemic lower limb. Curcumin, a monomer isolated from turmeric roots, has been demonstrated to have pro- and anti-angiogenic effects under different circumstances. Previous studies have indicated that curcumin treatment improves tissue repair and perfusion recovery in a mouse model of diabetic PAD. However, the effects of curcumin on PAD under non-diabetic conditions has remained elusive, In the present study, mice with PAD and a normal glycaemic profile were treated with curcumin, which improved perfusion recovery, increased capillary density and elevated microRNA (miR)-93 expression in ischemic muscle tissue. In cultured endothelial cells under simulated ischemia, curcumin improved endothelial cell viability and enhanced tube formation. However, following miR-93 knockdown using a microRNA inhibitor, endothelial cell tube formation was inhibited. Furthermore, in the presence of the miR-93 inhibitor, curcumin did not alter endothelial cell viability or tube formation. These results demonstrate that curcumin had beneficial effects in non-diabetic PAD by improving angiogenesis, which may have been achieved partially via the promotion of miR-93 expression.