Evaluation of a stepwise approach using microbiota analysis, species-specific qPCRs and culture for the diagnosis of lower respiratory tract infections.

van den Munckhof, Ellen H A; de Koning, Maurits N C; Quint, Wim G V; van Doorn, Leen-Jan; Leverstein-van Hall, Maurine A

van den Munckhof, Ellen H A; de Koning, Maurits N C; Quint, Wim G V; van Doorn, Leen-Jan; Leverstein-van Hall, Maurine A.

Affiliation

van den Munckhof EHA; DDL Diagnostic Laboratory, 2288 ER, Rijswijk, The Netherlands. ellen.van.den.munckhof@ddl.nl.
de Koning MNC; DDL Diagnostic Laboratory, 2288 ER, Rijswijk, The Netherlands.
Quint WGV; DDL Diagnostic Laboratory, 2288 ER, Rijswijk, The Netherlands.
van Doorn LJ; DDL Diagnostic Laboratory, 2288 ER, Rijswijk, The Netherlands.
Leverstein-van Hall MA; Department of Medical Microbiology, Haaglanden Medical Centre Bronovo, 2597 AX, The Hague, The Netherlands.

Eur J Clin Microbiol Infect Dis ; 38(4): 747-754, 2019 Apr.

Article in En | MEDLINE | ID: mdl-30788730

Subject(s)

Microbiota; Real-Time Polymerase Chain Reaction; Respiratory Tract Infections/diagnosis; Colony Count, Microbial; DNA, Bacterial/genetics; Haemophilus influenzae/genetics; Haemophilus influenzae/isolation & purification; Humans; RNA, Ribosomal, 16S/genetics; Respiratory Tract Infections/microbiology; Sequence Analysis, DNA; Species Specificity; Sputum/microbiology; Streptococcus/genetics; Streptococcus/isolation & purification; Whole Genome Sequencing

Key words

Culture; Diagnosis; Lower respiratory tract infection; Microbiota analysis; Quantitative real-time PCR; Sputum

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Full text: 1 Database: MEDLINE Main subject: Respiratory Tract Infections / Real-Time Polymerase Chain Reaction / Microbiota Type of study: Diagnostic_studies / Evaluation_studies / Prognostic_studies Limits: Humans Language: En Year: 2019 Type: Article

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