Methods and Strategies for Procurement, Isolation, Characterization, and Assessment of Senescence of Human Mesenchymal Stem Cells from Adipose Tissue.

Gaur, Meenakshi; Dobke, Marek; Lunyak, Victoria V

Gaur, Meenakshi; Dobke, Marek; Lunyak, Victoria V.

Affiliation

Gaur M; Aelan Cell Technologies, San Francisco, CA, USA.
Dobke M; Division of Plastic Surgery, University of California, San Diego, La Jolla, CA, USA. mdobke@ucsd.edu.
Lunyak VV; Aelan Cell Technologies, San Francisco, CA, USA. vlunyak@aelanCT.com.

Methods Mol Biol ; 2045: 37-92, 2019.

Article in En | MEDLINE | ID: mdl-30838605

Subject(s)

Adipose Tissue/metabolism; Adult Stem Cells/metabolism; Cell Culture Techniques/methods; Cell Differentiation/physiology; Cell Proliferation/physiology; Cellular Senescence/physiology; Mesenchymal Stem Cells/metabolism; Adipose Tissue/cytology; Adipose Tissue/growth & development; Adipose Tissue/surgery; Adult Stem Cells/cytology; Adult Stem Cells/physiology; Aging/genetics; Aging/metabolism; Aging/physiology; Cell Differentiation/genetics; Cell Proliferation/genetics; Cells, Cultured; Cellular Senescence/genetics; Cryopreservation; Flow Cytometry; Fluorescent Antibody Technique; Humans; Immunophenotyping; Mesenchymal Stem Cells/cytology; Regenerative Medicine; Signal Transduction/genetics; Tissue Donors; Workflow

Key words

Adipose tissue; Differentiation; Fat procurement; Mesenchymal stem cells; Proliferation; Senescence; Senescence messaging secretome (SMS); Stromal vascular fraction

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Full text: 1 Database: MEDLINE Main subject: Cell Differentiation / Adipose Tissue / Cellular Senescence / Cell Culture Techniques / Cell Proliferation / Adult Stem Cells / Mesenchymal Stem Cells Type of study: Guideline Limits: Humans Language: En Year: 2019 Type: Article

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