Critical evaluation of the ADCC test.

ABSTRACT

The activity of monocytes in the ADCC test against human red blood cells sensitized with anti-Rh antibodies is unquestionable, however, a participation of lymphocytes in this reaction is still controversial. The aim of this paper was to test the cytotoxic activity of monocytes and lymphocytes isolated from human peripheral blood against erythrocytes coated with anti-C + D antibodies of Rh system. Due to some individual changes in the activity of mononuclears observed in the ADCC test, it was of interest to test how this activity changes with time, in individual healthy subjects. Both types of mononuclears were observed to damage the sensitized non-treated as well as papain-treated red cells. The cytotoxic activity of these cells, however, showed substantial deviation in the same person.