Efficient chiral synthesis by Saccharomyces cerevisiae spore encapsulation of Candida parapsilosis Glu228Ser/(S)-carbonyl reductase II and Bacillus sp. YX-1 glucose dehydrogenase in organic solvents.
Microb Cell Fact
; 18(1): 87, 2019 May 20.
Article
in En
| MEDLINE
| ID: mdl-31109314
ABSTRACT
BACKGROUND:
Saccharomyces cerevisiae AN120 osw2∆ spores were used as a host with good resistance to unfavorable environment. This work was undertaken to develop a new yeast spore-encapsulation of Candida parapsilosis Glu228Ser/(S)-carbonyl reductase II and Bacillus sp. YX-1 glucose dehydrogenase for efficient chiral synthesis in organic solvents.RESULTS:
The spore microencapsulation of E228S/SCR II and GDH in S. cerevisiae AN120 osw2∆ catalyzed (R)-phenylethanol in a good yield with an excellent enantioselectivity (up to 99%) within 4 h. It presented good resistance and catalytic functions under extreme temperature and pH conditions. The encapsulation produced several chiral products with over 70% yield and over 99% enantioselectivity in ethyl acetate after being recycled for 4-6 times. It increased substrate concentration over threefold and reduced the reaction time two to threefolds compared to the recombinant Escherichia coli containing E228S and glucose dehydrogenase.CONCLUSIONS:
This work first described sustainable enantioselective synthesis without exogenous cofactors in organic solvents using yeast spore-microencapsulation of coupled alcohol dehydrogenases.Key words
Full text:
1
Database:
MEDLINE
Main subject:
Saccharomyces cerevisiae
/
Spores, Fungal
/
Bacillus
/
Glucose 1-Dehydrogenase
/
Alcohol Oxidoreductases
/
Drug Compounding
/
Candida parapsilosis
Language:
En
Year:
2019
Type:
Article