Immuno-localization of ESCRT Proteins in Virus-Infected Cells by Fluorescence and Electron Microscopy.

Tabata, Keisuke; Nara, Atsuki; Omori, Hiroko; Morita, Eiji

Tabata, Keisuke; Nara, Atsuki; Omori, Hiroko; Morita, Eiji.

Affiliation

Tabata K; Department of Infectious Diseases, Molecular Virology, Heidelberg University, Heidelberg, Germany.
Nara A; Nagahama Institute of Bio-Science and Technology, Nagahama, Japan.
Omori H; Core Instrumentation Facility, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
Morita E; Department of Biochemistry and Molecular Biology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Japan. moritae@hirosaki-u.ac.jp.

Methods Mol Biol ; 1998: 73-92, 2019.

Article in En | MEDLINE | ID: mdl-31250295

Subject(s)

Biological Assay/methods; Endoplasmic Reticulum/ultrastructure; Endosomal Sorting Complexes Required for Transport/metabolism; Microscopy, Electron, Transmission/methods; Molecular Imaging/methods; Animals; Cell Culture Techniques/methods; Cell Line; Dengue Virus/immunology; Encephalitis Virus, Japanese/immunology; Endoplasmic Reticulum/metabolism; Endoplasmic Reticulum/virology; Endosomal Sorting Complexes Required for Transport/chemistry; Endosomal Sorting Complexes Required for Transport/immunology; Gold/chemistry; Humans; Mesocricetus; Metal Nanoparticles/chemistry; Microscopy, Fluorescence/methods; Microscopy, Immunoelectron/methods; Staining and Labeling/methods; Virion/chemistry

Key words

Conventional transmission EM (TEM); Correlative light microscopy and electron microscopy (CLEM); Dengue virus (DENV); Fluorescence light microscopy; Immunoelectron microscopy; Japanese encephalitis virus (JEV); Positive strand RNA virus

Fulltext

XML

PubMed Links

Search on Google

Full text: 1 Database: MEDLINE Main subject: Biological Assay / Microscopy, Electron, Transmission / Endoplasmic Reticulum / Endosomal Sorting Complexes Required for Transport / Molecular Imaging Limits: Animals / Humans Language: En Year: 2019 Type: Article

Fulltext

XML

PubMed Links

Search on Google