4'-Guanidinium-modified siRNA: a molecular tool to control RNAi activity through RISC priming and selective antisense strand loading.

Nawale, Ganesh N; Bahadorikhalili, Saeed; Sengupta, Pallabi; Kadekar, Sandeep; Chatterjee, Subhrangsu; Varghese, Oommen P

Nawale, Ganesh N; Bahadorikhalili, Saeed; Sengupta, Pallabi; Kadekar, Sandeep; Chatterjee, Subhrangsu; Varghese, Oommen P.

Affiliation

Nawale GN; Translational Chemical Biology Laboratory, Division of Polymer Chemistry, Department of Chemistry-Ångström, Uppsala University, Uppsala, Sweden. oommen.varghese@kemi.uu.se.

Chem Commun (Camb) ; 55(62): 9112-9115, 2019 Jul 30.

Article in En | MEDLINE | ID: mdl-31298670

ABSTRACT

ABSTRACT

We designed novel 4'-C-guanidinocarbohydrazidomethyl-5-methyl uridine (GMU) modified small interfering RNA (siRNA) and evaluated its biophysical and biochemical properties. Incorporation of GMU units significantly increased the thermodynamic stability as well as the enzymatic stability against nucleases in human serum. A gene silencing experiment indicated that GMU modfied siRNA (siRNA6) resulted in ≈4.9-fold more efficient knockdown than unmodified siRNA.

Subject(s)

Guanidine/chemistry; RNA Interference; RNA, Small Interfering/chemistry; RNA, Small Interfering/metabolism; Guanidine/analogs & derivatives; Models, Molecular; RNA, Small Interfering/genetics; Ribonucleases/blood; Ribonucleases/metabolism; Thermodynamics

Fulltext

XML

PubMed Links

Search on Google

Full text: 1 Database: MEDLINE Main subject: Guanidine / RNA, Small Interfering / RNA Interference Language: En Year: 2019 Type: Article

Fulltext

XML

PubMed Links

Search on Google

Full text: 1 Database: MEDLINE Main subject: Guanidine / RNA, Small Interfering / RNA Interference Language: En Year: 2019 Type: Article