Evaluation of a Commercial Colloidal Gold Assay for Detection of Influenza A and B Virus in Children's Respiratory Specimens.
Fetal Pediatr Pathol
; 39(2): 93-98, 2020 Apr.
Article
in En
| MEDLINE
| ID: mdl-31304835
ABSTRACT
Objective:
To evaluate the clinical diagnostic value of colloidal gold assay for detection of influenza A and B virus.Methods:
Results were compared for colloidal gold assay for influenza antigen detection and real-time RT-PCR for RNA detection.Results:
By performing the colloidal gold assay, the positive rate was 25.67% (105/409) for influenza A virus and 8.56% (35/409) for influenza B virus. While tested by real-time RT-PCR, 107(26.2%) were positive for influenza A virus and 35(8.6%) for influenza B virus. Using real-time RT-PCR as the gold standard, the sensitivity and specificity of the colloidal gold assay were 84.1% and 95.0%, respectively, for influenza A virus and 85.7% and 98.7% for influenza B virus.Conclusion:
This colloidal gold assay may be one of the viable tools for the rapid screening for influenza viruses in an outpatient clinical practice.Key words
Full text:
1
Database:
MEDLINE
Main subject:
Influenza A virus
/
Influenza B virus
/
Gold Colloid
Type of study:
Diagnostic_studies
/
Evaluation_studies
Limits:
Child
/
Child, preschool
/
Humans
Language:
En
Year:
2020
Type:
Article