A rapid fluorimetric test for lysozyme with purified fluorescamine-labelled peptidoglycan.

Moser, I; Pittner, F; Dworsky, P

Moser, I; Pittner, F; Dworsky, P.

Affiliation

Moser I; Institut für Allgemeine Biochemie, Ludwig Boltzmann Forschungsstelle für Biochemie, Vienna, Austria.

J Biochem Biophys Methods ; 17(4): 249-52, 1988 Dec.

Article in En | MEDLINE | ID: mdl-3149658

ABSTRACT

ABSTRACT

A sensitive and rapid fluorometric lysozyme assay is described. It is based on the hydrolysis of fluorescamine-labelled peptidoglycan from Micrococcus luteus cell walls. Lysozyme levels as low as 0.1 microgram can be detected.

Subject(s)

Muramidase/analysis; Bacillus subtilis; Fluorescamine; Indicators and Reagents; Micrococcus; Peptidoglycan/isolation & purification; Spectrometry, Fluorescence/methods

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