Ultrahigh-Density Screens for Genome-Wide Yeast EMAPs in a Single Plate.
Methods Mol Biol
; 2049: 73-85, 2019.
Article
in En
| MEDLINE
| ID: mdl-31602605
ABSTRACT
Systematic measurements of genetic interactions have been used to classify gene functions and to categorize genes into protein complexes, functional pathways and biological processes. This protocol describes how to perform a high-throughput genetic interaction screen in S. cerevisiae using a variant of epistatic miniarray profiles (E-MAP) in which the fitnesses of 6144 colonies are measured simultaneously. We also describe the computational methods to analyze the resulting data.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Saccharomyces cerevisiae
/
Genome, Fungal
/
Epistasis, Genetic
Language:
En
Year:
2019
Type:
Article