[Development and assessment of a reagent kit for RNA detection of CrimeanCongo hemorrhagic fever virus with using reverse transcription loop-mediated isothermal amplification method.]
Klin Lab Diagn
; 64(9): 571-577, 2019.
Article
in Ru
| MEDLINE
| ID: mdl-31610111
ABSTRACT
This study presents the results of laboratory trials of the reagent kit for the rapid detection of RNA of the Crimean-Congo hemorrhagic fever virus (CCHFV) using loop-mediated isothermal amplification with reverse transcription (RT-LAMP). The developed RT-LAMP reagent kit was used to detect the CCHFV and showed a sensitivity of 103 GE/ml of viral RNA, which is sufficient for detection of the CCHFV in the early stage of human infections. The kit showed high specificity and no cross-reactivity with viral panel from the State collection of viruses of the FBRI SRC VB «Vector¼ (arboviruses and hemorrhagic fever viruses). Laboratory trials of the RT-LAMP kit are showed a high analytical and diagnostic sensitivity and specificity for RNA detection of the CCHFV and high speed of the analysis (60-70 min with sample preparation) compared to real-time PCR. Approbation of the kit field version has showed the possibility of setting the RT-LAMP reaction and viral RNA detection without the using of analytical equipments.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Reagent Kits, Diagnostic
/
Hemorrhagic Fever Virus, Crimean-Congo
/
Nucleic Acid Amplification Techniques
/
Hemorrhagic Fever, Crimean
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
Ru
Year:
2019
Type:
Article