RNA Fragmentation and Sequencing (RF-Seq): Cost-Effective, Time-Efficient, and High-Throughput 3' mRNA Sequencing Library Construction in a Single Tube.

Veeranagouda, Yaligara; Remaury, Anne; Guillemot, Jean-Claude; Didier, Michel

Veeranagouda, Yaligara; Remaury, Anne; Guillemot, Jean-Claude; Didier, Michel.

Affiliation

Veeranagouda Y; Molecular Biology and Genomics, Translational Sciences, Sanofi R&D, Chilly-Mazarin, France.
Remaury A; Proteomics, Translational Sciences, Sanofi R&D, Chilly-Mazarin, France.
Guillemot JC; Proteomics, Translational Sciences, Sanofi R&D, Chilly-Mazarin, France.
Didier M; Molecular Biology and Genomics, Translational Sciences, Sanofi R&D, Chilly-Mazarin, France.

Curr Protoc Mol Biol ; 129(1): e109, 2019 12.

Article in En | MEDLINE | ID: mdl-31763778

Subject(s)

Gene Library; High-Throughput Nucleotide Sequencing/methods; Polymerase Chain Reaction/methods; RNA, Messenger/genetics; Sequence Analysis, RNA/methods; Humans; Membrane Proteins/blood; Membrane Proteins/genetics; Transcriptome

Key words

RF-Seq; RNA-Seq; barcode PCR; high-throughput assay; mRNA sequencing; next-generation sequencing; template switch oligo

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Full text: 1 Database: MEDLINE Main subject: RNA, Messenger / Gene Library / Polymerase Chain Reaction / Sequence Analysis, RNA / High-Throughput Nucleotide Sequencing Type of study: Health_economic_evaluation Limits: Humans Language: En Year: 2019 Type: Article

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