Optimized photo-stimulation of halorhodopsin for long-term neuronal inhibition.
BMC Biol
; 17(1): 95, 2019 11 27.
Article
in En
| MEDLINE
| ID: mdl-31775747
ABSTRACT
BACKGROUND:
Optogenetic silencing techniques have expanded the causal understanding of the functions of diverse neuronal cell types in both the healthy and diseased brain. A widely used inhibitory optogenetic actuator is eNpHR3.0, an improved version of the light-driven chloride pump halorhodopsin derived from Natronomonas pharaonis. A major drawback of eNpHR3.0 is related to its pronounced inactivation on a time-scale of seconds, which renders it unsuited for applications that require long-lasting silencing.RESULTS:
Using transgenic mice and Xenopus laevis oocytes expressing an eNpHR3.0-EYFP fusion protein, we here report optimized photo-stimulation techniques that profoundly increase the stability of eNpHR3.0-mediated currents during long-term photo-stimulation. We demonstrate that optimized photo-stimulation enables prolonged hyperpolarization and suppression of action potential discharge on a time-scale of minutes.CONCLUSIONS:
Collectively, our findings extend the utility of eNpHR3.0 to the long-lasting inhibition of excitable cells, thus facilitating the optogenetic dissection of neural circuits.Key words
Full text:
1
Database:
MEDLINE
Main subject:
Bacterial Proteins
/
Action Potentials
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Halorhodopsins
/
Optogenetics
/
Neurons
Limits:
Animals
Language:
En
Year:
2019
Type:
Article