Cell-free gene expression: an expanded repertoire of applications.
Nat Rev Genet
; 21(3): 151-170, 2020 03.
Article
in En
| MEDLINE
| ID: mdl-31780816
ABSTRACT
Cell-free biology is the activation of biological processes without the use of intact living cells. It has been used for more than 50 years across the life sciences as a foundational research tool, but a recent technical renaissance has facilitated high-yielding (grams of protein per litre), cell-free gene expression systems from model bacteria, the development of cell-free platforms from non-model organisms and multiplexed strategies for rapidly assessing biological design. These advances provide exciting opportunities to profoundly transform synthetic biology by enabling new approaches to the model-driven design of synthetic gene networks, the fast and portable sensing of compounds, on-demand biomanufacturing, building cells from the bottom up, and next-generation educational kits.
Full text:
1
Database:
MEDLINE
Main subject:
Gene Expression
/
Cell-Free System
Language:
En
Year:
2020
Type:
Article