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Towards real-time wide-field fluorescence lifetime imaging of 5-ALA labeled brain tumors with multi-tap CMOS cameras.
Reichert, David; Erkkilä, Mikael T; Holst, Gerhard; Hecker-Denschlag, Nancy; Wilzbach, Marco; Hauger, Christoph; Drexler, Wolfgang; Gesperger, Johanna; Kiesel, Barbara; Roetzer, Thomas; Unterhuber, Angelika; Widhalm, Georg; Leitgeb, Rainer A; Andreana, Marco.
Affiliation
  • Reichert D; Medical University of Vienna, Center for Medical Physics and Biomedical Engineering, 1090 Vienna, Austria.
  • Erkkilä MT; Medical University of Vienna, Christian Doppler Laboratory OPTRAMED, 1090 Vienna, Austria.
  • Holst G; These authors contributed equally to this work.
  • Hecker-Denschlag N; Medical University of Vienna, Center for Medical Physics and Biomedical Engineering, 1090 Vienna, Austria.
  • Wilzbach M; These authors contributed equally to this work.
  • Hauger C; PCO AG, Science and Research, 93309 Kelheim, Germany.
  • Drexler W; Carl Zeiss Meditec AG, 73447 Oberkochen, Germany.
  • Gesperger J; Carl Zeiss Meditec AG, 73447 Oberkochen, Germany.
  • Kiesel B; Carl Zeiss Meditec AG, 73447 Oberkochen, Germany.
  • Roetzer T; Medical University of Vienna, Center for Medical Physics and Biomedical Engineering, 1090 Vienna, Austria.
  • Unterhuber A; Medical University of Vienna, Center for Medical Physics and Biomedical Engineering, 1090 Vienna, Austria.
  • Widhalm G; General Hospital and Medical University of Vienna, Institute of Neurology, 1090 Vienna, Austria.
  • Leitgeb RA; General Hospital and Medical University of Vienna, Department of Neurosurgery, 1090 Vienna, Austria.
  • Andreana M; General Hospital and Medical University of Vienna, Institute of Neurology, 1090 Vienna, Austria.
Biomed Opt Express ; 11(3): 1598-1616, 2020 Mar 01.
Article in En | MEDLINE | ID: mdl-32206431
ABSTRACT
Fluorescence guided neurosurgery based on 5-aminolevulinic acid (5-ALA) has significantly increased maximal safe resections. Fluorescence lifetime imaging (FLIM) of 5-ALA could further boost this development by its increased sensitivity. However, neurosurgeons require real-time visual feedback which was so far limited in dual-tap CMOS camera based FLIM. By optimizing the number of phase frames required for reconstruction, we here demonstrate real-time 5-ALA FLIM of human high- and low-grade glioma with up to 12 Hz imaging rate over a wide field of view (11.0 x 11.0 mm). Compared to conventional fluorescence imaging, real-time FLIM offers enhanced contrast of weakly fluorescent tissue.
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Full text: 1 Database: MEDLINE Language: En Year: 2020 Type: Article
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Full text: 1 Database: MEDLINE Language: En Year: 2020 Type: Article