2,4-Dichlorophenoxyacetic acid functionalized gold nanoparticles: synthesis, characterization and biological effects.
J Mater Chem B
; 2(21): 3299-3305, 2014 Jun 07.
Article
in En
| MEDLINE
| ID: mdl-32261592
ABSTRACT
Understanding and visualizing the biodistribution of agricultural chemicals inside cells and living plants is very important for enhancing targeting and changing the application approaches of chemicals. Here, a novel material was synthesized through 2,4-dichlorophenoxyacetic acid functionalized small gold nanoparticles (2,4-D-MP-Au NPs). The successful modification of Au NPs (4.46 ± 0.70 nm) was ascertained by UV-vis, TEM, FTIR and XPS. TGA data revealed about 1197 molecules of 2,4-D were coupled to the surface of one Au nanoparticle, which was sufficient for bioapplications. The optical imaging of 2,4-D-MP-Au NPs inside BY-2 cells was directly examined, revealing that 2,4-D-MP-Au NPs could be internalized in BY-2 cells by the two-photo microscopy and endocytosis, as the internalization mechanism was energy dependent for 2,4-D-MP-Au NPs. Furthermore, the biodistribution of 2,4-D-MP-Au NPs in Ricinus cotyledons was measured, revealing that 2,4-D-MP-Au NPs could enter into mesophyll cells of Ricinus cotyledons; the cell recognition was enhanced after 2,4-D conjugated Au NPs. These results indicate that the conjugates have great potential for applications on bioimaging and biolabeling for agricultural chemicals in plant physiology.
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Database:
MEDLINE
Language:
En
Year:
2014
Type:
Article