Preparation of Recombinant Membrane Proteins from Pichia pastoris for Molecular Investigations.

Guyot, Lucile; Hartmann, Lucie; Mohammed-Bouteben, Sarah; Caro, Lydia; Wagner, Renaud

Guyot, Lucile; Hartmann, Lucie; Mohammed-Bouteben, Sarah; Caro, Lydia; Wagner, Renaud.

Affiliation

Guyot L; IMPReSs Facility, Biotechnology and Cell Signaling UMR 7242, CNRS-University of Strasbourg, Illkirch, Cedex, France.
Hartmann L; NovAliX, Illkirch, France.
Mohammed-Bouteben S; IMPReSs Facility, Biotechnology and Cell Signaling UMR 7242, CNRS-University of Strasbourg, Illkirch, Cedex, France.
Caro L; IMPReSs Facility, Biotechnology and Cell Signaling UMR 7242, CNRS-University of Strasbourg, Illkirch, Cedex, France.
Wagner R; IMPReSs Facility, Biotechnology and Cell Signaling UMR 7242, CNRS-University of Strasbourg, Illkirch, Cedex, France.

Curr Protoc Protein Sci ; 100(1): e104, 2020 06.

Article in En | MEDLINE | ID: mdl-32289210

Subject(s)

Membrane Lipids; Membrane Proteins; Nanoparticles/chemistry; Saccharomycetales; Membrane Lipids/chemistry; Membrane Lipids/metabolism; Membrane Proteins/biosynthesis; Membrane Proteins/chemistry; Membrane Proteins/genetics; Recombinant Proteins/biosynthesis; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Saccharomycetales/genetics; Saccharomycetales/metabolism

Key words

Pichia pastoris; detergent; extraction; integral membrane proteins; lipid particles reconstitution; protoplast; purification; recombinant expression

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Full text: 1 Database: MEDLINE Main subject: Saccharomycetales / Nanoparticles / Membrane Lipids / Membrane Proteins Language: En Year: 2020 Type: Article

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Full text: 1 Database: MEDLINE Main subject: Saccharomycetales / Nanoparticles / Membrane Lipids / Membrane Proteins Language: En Year: 2020 Type: Article