The Vif protein of caprine arthritis encephalitis virus inhibits interferon production.
Arch Virol
; 165(7): 1557-1567, 2020 Jul.
Article
in En
| MEDLINE
| ID: mdl-32356187
ABSTRACT
Caprine arthritis-encephalitis (CAE) is a chronic progressive infectious disease caused by caprine arthritis-encephalitis virus (CAEV) that seriously threatens the goat industry. Chronic infection and life-long multi-tissue inflammation are the typical features of the disease. Innate antiviral immunity is essential for the host defense system that rapidly recognizes and eliminates invading viruses. Interferon ß (IFN-ß) is important for innate immunity and regulates immunity against a broad spectrum of viruses. To investigate the details of the IFN-ß response to CAEV infection, the effects of six viral proteins and the molecular mechanisms by which they affect IFN-ß production were analyzed. Overexpression of DU and Vif promote virus proliferation and inhibit the production of IFN-ß. qRT-PCR and luciferase reporter assays showed that overexpression of Vif inhibits the expression of luciferase under the control of the ISRE, NF-κB or IFN-ß promoter but does not affect the expression of IFN-ß activated by IRF3, indicating that Vif negatively regulates IFN-ß production by affecting upstream signal transduction of IRF3. Amino acids 149-164 of Vif were found to be necessary for the inhibitory effect of IFN-ß production. Our results indicate that CAEV evades surveillance and clearance by intracellular innate immunity by downregulating IFN-ß production.
Full text:
1
Database:
MEDLINE
Main subject:
Goat Diseases
/
Gene Products, vif
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Lentivirus Infections
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Interferon-beta
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Arthritis-Encephalitis Virus, Caprine
Limits:
Animals
Language:
En
Year:
2020
Type:
Article