Time-Resolved Fluorescence Anisotropy of a Molecular Rotor Resolves Microscopic Viscosity Parameters in Complex Environments.

Steinmark, I Emilie; Chung, Pei-Hua; Ziolek, Robert M; Cornell, Bethan; Smith, Paul; Levitt, James A; Tregidgo, Carolyn; Molteni, Carla; Yahioglu, Gokhan; Lorenz, Christian D; Suhling, Klaus.

Affiliation

Steinmark IE; Department of Physics, King's College London, UK.
Chung PH; Department of Physics, King's College London, UK.
Ziolek RM; Department of Physics, King's College London, UK.
Cornell B; Department of Physics, King's College London, UK.
Smith P; Department of Physics, King's College London, UK.
Levitt JA; Randall Centre for Cell & Molecular Biophysics, King's College London, UK.
Tregidgo C; Department of Physics, King's College London, UK.
Molteni C; Genomics England, London, EC1M 6BQ, UK.
Yahioglu G; Department of Physics, King's College London, UK.
Lorenz CD; Antikor Biopharma Ltd., Stevenage, SG1 2FX, UK.
Suhling K; Department of Physics, King's College London, UK.

Small ; 16(22): e1907139, 2020 06.

Article in En | MEDLINE | ID: mdl-32363742

Subject(s)

Fluorescent Dyes; Optical Imaging; Anisotropy; Fluorescence Polarization; Lipids; Viscosity

Key words

lipid droplets; molecular dynamics simulations; molecular rotors; time-resolved fluorescence anisotropy; viscosity imaging time-correlated single photon counting (TCSPC)