Expansion Microscopy for Beginners: Visualizing Microtubules in Expanded Cultured HeLa Cells.

Zhang, Chi; Kang, Jeong Seuk; Asano, Shoh M; Gao, Ruixuan; Boyden, Edward S

Zhang, Chi; Kang, Jeong Seuk; Asano, Shoh M; Gao, Ruixuan; Boyden, Edward S.

Affiliation

Zhang C; Media Lab, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts.
Kang JS; McGovern Institute, MIT, Cambridge, Massachusetts.
Asano SM; John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts.
Gao R; Media Lab, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts.
Boyden ES; McGovern Institute, MIT, Cambridge, Massachusetts.

Curr Protoc Neurosci ; 92(1): e96, 2020 06.

Article in En | MEDLINE | ID: mdl-32497404

Subject(s)

Microscopy, Fluorescence; Microtubules/metabolism; Tissue Preservation; Cells, Cultured; HeLa Cells; Humans; Microscopy, Fluorescence/methods; RNA/metabolism; Tissue Preservation/methods

Key words

HeLa cells; antibody; beginner; clearing; expansion microscopy; fluorescence microscopy; hydrogel; imaging; immunocytochemistry; immunohistochemistry; microscopy; microtubules; super-resolution microscopy

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Full text: 1 Database: MEDLINE Main subject: Tissue Preservation / Microscopy, Fluorescence / Microtubules Limits: Humans Language: En Year: 2020 Type: Article

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Full text: 1 Database: MEDLINE Main subject: Tissue Preservation / Microscopy, Fluorescence / Microtubules Limits: Humans Language: En Year: 2020 Type: Article