A SARS-CoV-2 Infection Model in Mice Demonstrates Protection by Neutralizing Antibodies.
Cell
; 182(3): 744-753.e4, 2020 08 06.
Article
in En
| MEDLINE
| ID: mdl-32553273
ABSTRACT
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a pandemic with millions of human infections. One limitation to the evaluation of potential therapies and vaccines to inhibit SARS-CoV-2 infection and ameliorate disease is the lack of susceptible small animals in large numbers. Commercially available laboratory strains of mice are not readily infected by SARS-CoV-2 because of species-specific differences in their angiotensin-converting enzyme 2 (ACE2) receptors. Here, we transduced replication-defective adenoviruses encoding human ACE2 via intranasal administration into BALB/c mice and established receptor expression in lung tissues. hACE2-transduced mice were productively infected with SARS-CoV-2, and this resulted in high viral titers in the lung, lung pathology, and weight loss. Passive transfer of a neutralizing monoclonal antibody reduced viral burden in the lung and mitigated inflammation and weight loss. The development of an accessible mouse model of SARS-CoV-2 infection and pathogenesis will expedite the testing and deployment of therapeutics and vaccines.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Pneumonia, Viral
/
Coronavirus Infections
/
Disease Models, Animal
/
Antibodies, Neutralizing
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Betacoronavirus
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Antibodies, Monoclonal
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Antibodies, Viral
Limits:
Animals
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Female
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Humans
/
Male
Language:
En
Year:
2020
Type:
Article