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Variable expression and silencing of CRISPR-Cas9 targeted transgenes identifies the AAVS1 locus as not an entirely safe harbour.
Bhagwan, Jamie R; Collins, Emma; Mosqueira, Diogo; Bakar, Mine; Johnson, Benjamin B; Thompson, Alexander; Smith, James G W; Denning, Chris.
Affiliation
  • Bhagwan JR; Department of Stem Cells, Tissue Engineering and Modelling, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, NG7 2RD, UK.
  • Collins E; Department of Stem Cells, Tissue Engineering and Modelling, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, NG7 2RD, UK.
  • Mosqueira D; Department of Stem Cells, Tissue Engineering and Modelling, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, NG7 2RD, UK.
  • Bakar M; Department of Stem Cells, Tissue Engineering and Modelling, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, NG7 2RD, UK.
  • Johnson BB; Department of Stem Cells, Tissue Engineering and Modelling, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, NG7 2RD, UK.
  • Thompson A; Department of Stem Cells, Tissue Engineering and Modelling, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, NG7 2RD, UK.
  • Smith JGW; Faculty of Medicine and Health Sciences, Norwich Medical School, University of East Anglia, Norwich Research Park, Norwich, NR4 7UQ, UK.
  • Denning C; Department of Stem Cells, Tissue Engineering and Modelling, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, NG7 2RD, UK.
F1000Res ; 8: 1911, 2019.
Article in En | MEDLINE | ID: mdl-32789000

Full text: 1 Database: MEDLINE Main subject: Gene Targeting / Transgenes / Gene Silencing / Induced Pluripotent Stem Cells / Genetic Loci / CRISPR-Cas Systems Type of study: Prognostic_studies Limits: Humans Language: En Year: 2019 Type: Article

Full text: 1 Database: MEDLINE Main subject: Gene Targeting / Transgenes / Gene Silencing / Induced Pluripotent Stem Cells / Genetic Loci / CRISPR-Cas Systems Type of study: Prognostic_studies Limits: Humans Language: En Year: 2019 Type: Article