Advances in Antibody Preparation Techniques for Immunoassays of Total Aflatoxin in Food.
Molecules
; 25(18)2020 Sep 09.
Article
in En
| MEDLINE
| ID: mdl-32916811
ABSTRACT
Aflatoxin (AF) contamination is a major concern in the food and feed industry because of its prevalence and toxicity. Improved aflatoxin detection methods are still needed. Immunoassays are an important method for total aflatoxin (TAF) analysis in food due to its technical advantages such as high specificity, sensitivity, and simplicity, but require high-quality antibodies. Here, we first review the three ways to prepare high-quality antibodies for TAF immunoassay, second, compare the advantages and disadvantages of antigen synthesis methods for B-group and G-group aflatoxins, and third, describe the status of novel genetic engineering antibodies. This review can provide new methods and ideas for the development of TAF immunoassays.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Immunoassay
/
Food Contamination
/
Aflatoxins
/
Food Analysis
Type of study:
Diagnostic_studies
/
Risk_factors_studies
Limits:
Animals
/
Humans
Language:
En
Year:
2020
Type:
Article