Your browser doesn't support javascript.
loading
Streptomyces hygroscopicus UFPEDA 3370: A valuable source of the potent cytotoxic agent nigericin and its evaluation against human colorectal cancer cells.
Garcia-Princival, Iza Mirela Rodini; Princival, Jefferson Luiz; Dias da Silva, Emmanuel; de Arruda Lima, Sandrine Maria; Carregosa, Jhonattas Carvalho; Wisniewski, Alberto; de Lucena, Caio Cézar Oliveira; Halwass, Fernando; Alves Franca, José Adonias; Ferreira, Luiz Felipe Gomes Rebello; Hernandes, Marcelo Zaldini; Saraiva, Karina Lidianne Alcântara; Peixoto, Christina Alves; Baratte, Blandine; Robert, Thomas; Bach, Stéphane; Gomes, Dayene Correia; Guedes Paiva, Patricia Maria; Marchand, Pascal; Rodrigues, Maria do Desterro; Gonçalves da Silva, Teresinha.
Affiliation
  • Garcia-Princival IMR; Departamento de Antibióticos, Rua Prof. Moraes Rego, 1235, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil.
  • Princival JL; Departamento de Química Fundamental, Av. Jornalista Anibal Fernandes, s/n, Universidade Federal de Pernambuco, Recife, Pernambuco, 50740-560, Brazil.
  • Dias da Silva E; Departamento de Química Fundamental, Av. Jornalista Anibal Fernandes, s/n, Universidade Federal de Pernambuco, Recife, Pernambuco, 50740-560, Brazil.
  • de Arruda Lima SM; Departamento de Antibióticos, Rua Prof. Moraes Rego, 1235, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil.
  • Carregosa JC; Departamento de Química, Av. Marechal Rondon, s/n, Universidade Federal de Sergipe, Aracaju, Sergipe, 49100-000, Brazil.
  • Wisniewski A; Departamento de Química, Av. Marechal Rondon, s/n, Universidade Federal de Sergipe, Aracaju, Sergipe, 49100-000, Brazil.
  • de Lucena CCO; Departamento de Antibióticos, Rua Prof. Moraes Rego, 1235, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil.
  • Halwass F; Departamento de Química Fundamental, Av. Jornalista Anibal Fernandes, s/n, Universidade Federal de Pernambuco, Recife, Pernambuco, 50740-560, Brazil.
  • Alves Franca JA; Departamento de Química Fundamental, Av. Jornalista Anibal Fernandes, s/n, Universidade Federal de Pernambuco, Recife, Pernambuco, 50740-560, Brazil.
  • Ferreira LFGR; Laboratório de Química Teórica Medicinal (LQTM), Departamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco, Av. Prof. Artur de Sá - Cidade Universitária, Recife, PE, 50740-521, Brazil.
  • Hernandes MZ; Laboratório de Química Teórica Medicinal (LQTM), Departamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco, Av. Prof. Artur de Sá - Cidade Universitária, Recife, PE, 50740-521, Brazil.
  • Saraiva KLA; Laboratório de Ultraestrutura. Instituto Aggeu Magalhães (IAM), Fundação Oswaldo Cruz (FIOCRUZ), Recife, PE, Brazil.
  • Peixoto CA; Laboratório de Ultraestrutura. Instituto Aggeu Magalhães (IAM), Fundação Oswaldo Cruz (FIOCRUZ), Recife, PE, Brazil; Instituto de Ciências e Tecnologia em Neuroimunomodulação (INCT-NIM), Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, RJ, Brazil.
  • Baratte B; Sorbonne Université, CNRS, UMR8227, Integrative Biology of Marine Models Laboratory (LBI2M), Station Biologique de Roscoff, F-29680, Roscoff, France; Sorbonne Université, CNRS, FR2424, Kinase Inhibitor Specialized Screening Facility - KISSf, Station Biologique, F-29688, Roscoff, France. Electronic a
  • Robert T; Sorbonne Université, CNRS, UMR8227, Integrative Biology of Marine Models Laboratory (LBI2M), Station Biologique de Roscoff, F-29680, Roscoff, France; Sorbonne Université, CNRS, FR2424, Kinase Inhibitor Specialized Screening Facility - KISSf, Station Biologique, F-29688, Roscoff, France.
  • Bach S; Sorbonne Université, CNRS, UMR8227, Integrative Biology of Marine Models Laboratory (LBI2M), Station Biologique de Roscoff, F-29680, Roscoff, France; Sorbonne Université, CNRS, FR2424, Kinase Inhibitor Specialized Screening Facility - KISSf, Station Biologique, F-29688, Roscoff, France. Electronic a
  • Gomes DC; Departamento de Bioquímica, Rua Prof. Moraes Rego, SN, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-420, Brazil.
  • Guedes Paiva PM; Departamento de Bioquímica, Rua Prof. Moraes Rego, SN, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-420, Brazil.
  • Marchand P; Universite de Nantes, Cibles et médicaments des infections et du cancer, IICiMed, EA 1155, Nantes, F-44000, France.
  • Rodrigues MDD; Departamento de Antibióticos, Rua Prof. Moraes Rego, 1235, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil.
  • Gonçalves da Silva T; Departamento de Antibióticos, Rua Prof. Moraes Rego, 1235, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil. Electronic address: teresinha.goncalves@pq.cnpq.br.
Chem Biol Interact ; 333: 109316, 2021 Jan 05.
Article in En | MEDLINE | ID: mdl-33285127
ABSTRACT
Streptomyces hygroscopicus UFPEDA 3370 was fermented in submerged cultivation and the biomass extract was partitioned, obtaining a fraction purified named EB1. After purification of EB1 fraction, nigericin free acid was obtained and identified. Nigericin presented cytotoxic activity against several cancer cell lines, being most active against HL-60 (human leukemia) and HCT-116 (human colon carcinoma) cell lines, presenting IC50 and (IS) values 0.0014 µM, (30.0) and 0.0138 µM (3.0), respectively. On HCT-116, nigericin caused apoptosis and autophagy. In this study, nigericin was also screened both in vitro and in silico against a panel of cancer-related kinases. Nigericin was able to inhibit both JAK3 and GSK-3ß kinases in vitro and its binding affinities were mapped through the intermolecular interactions with each target in silico.
Subject(s)
Key words
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Database: MEDLINE Main subject: Streptomyces / Colorectal Neoplasms / Protein Kinase Inhibitors / Nigericin / Antineoplastic Agents Limits: Humans Language: En Year: 2021 Type: Article
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Database: MEDLINE Main subject: Streptomyces / Colorectal Neoplasms / Protein Kinase Inhibitors / Nigericin / Antineoplastic Agents Limits: Humans Language: En Year: 2021 Type: Article