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Generation of Nonmosaic, Two-Pore Channel 2 Biallelic Knockout Pigs in One Generation by CRISPR-Cas9 Microinjection Before Oocyte Insemination.
Navarro-Serna, Sergio; Hachem, Alaa; Canha-Gouveia, Analuce; Hanbashi, Ali; Garrappa, Gabriela; Lopes, Jordana S; París-Oller, Evelyne; Sarrías-Gil, Lucía; Flores-Flores, Cesar; Bassett, Andrew; Sánchez, Raul; Bermejo-Álvarez, Pablo; Matás, Carmen; Romar, Raquel; Parrington, John; Gadea, Joaquín.
Affiliation
  • Navarro-Serna S; Department of Physiology, Faculty of Veterinary, University of Murcia, Campus Mare Nostrum, Murcia, Spain.
  • Hachem A; IMIB-Arrixaca, Murcia, Spain.
  • Canha-Gouveia A; Department of Pharmacology, University of Oxford, Oxford, United Kingdom.
  • Hanbashi A; Department of Anatomy, College of Veterinary Medicine, University of Al Qadisiyah, Al Diwaniyah, Iraq.
  • Garrappa G; Department of Physiology, Faculty of Veterinary, University of Murcia, Campus Mare Nostrum, Murcia, Spain.
  • Lopes JS; IMIB-Arrixaca, Murcia, Spain.
  • París-Oller E; Department of Pharmacology, University of Oxford, Oxford, United Kingdom.
  • Sarrías-Gil L; Department of Pharmacology, College of Pharmacy, Jazan University, Jazan, Saudi Arabia.
  • Flores-Flores C; Department of Physiology, Faculty of Veterinary, University of Murcia, Campus Mare Nostrum, Murcia, Spain.
  • Bassett A; Department of Physiology, Faculty of Veterinary, University of Murcia, Campus Mare Nostrum, Murcia, Spain.
  • Sánchez R; IMIB-Arrixaca, Murcia, Spain.
  • Bermejo-Álvarez P; Department of Physiology, Faculty of Veterinary, University of Murcia, Campus Mare Nostrum, Murcia, Spain.
  • Matás C; IMIB-Arrixaca, Murcia, Spain.
  • Romar R; Department of Physiology, Faculty of Veterinary, University of Murcia, Campus Mare Nostrum, Murcia, Spain.
  • Parrington J; Molecular Biology Section, Scientific and Technical Research Area (ACTI), University of Murcia, Murcia, Spain.
  • Gadea J; Gene Editing and Cellular Research and Development, Wellcome Sanger Institute, Cambridge, United Kingdom.
CRISPR J ; 4(1): 132-146, 2021 02.
Article in En | MEDLINE | ID: mdl-33616447
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Full text: 1 Database: MEDLINE Main subject: Oocytes / Swine / Gene Knockout Techniques / CRISPR-Cas Systems / Insemination / Microinjections Limits: Animals Language: En Year: 2021 Type: Article
Fulltext
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PubMed Links
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Full text: 1 Database: MEDLINE Main subject: Oocytes / Swine / Gene Knockout Techniques / CRISPR-Cas Systems / Insemination / Microinjections Limits: Animals Language: En Year: 2021 Type: Article