Your browser doesn't support javascript.
loading
Reflex Detection of Ciprofloxacin Resistance in Neisseria gonorrhoeae by Use of the SpeeDx ResistancePlus GC Assay.
Lee, Darren Y J; Ashcroft, Melinda M; Chow, Eric P F; Sait, Michelle; De Petra, Vesna; Tschaepe, Marlene; Lange, Sigrid; Taiaroa, George; Bradshaw, Catriona S; Whiley, David M; Fairley, Christopher K; Howden, Benjamin P; Chen, Marcus Y; Pasricha, Shivani; Williamson, Deborah A.
Affiliation
  • Lee DYJ; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia.
  • Ashcroft MM; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia.
  • Chow EPF; Melbourne Sexual Health Centre, Alfred Health, Carlton, Victoria, Australia.
  • Sait M; Central Clinical School, Monash University, Melbourne, Victoria, Australia.
  • De Petra V; Centre for Epidemiology and Biostatistics, Melbourne School of Population and Global Health, The University of Melbourne, Melbourne, Victoria, Australia.
  • Tschaepe M; Microbiological Diagnostic Unit Public Health Laboratory, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia.
  • Lange S; Microbiological Diagnostic Unit Public Health Laboratory, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia.
  • Taiaroa G; Microbiological Diagnostic Unit Public Health Laboratory, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia.
  • Bradshaw CS; Microbiological Diagnostic Unit Public Health Laboratory, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia.
  • Whiley DM; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia.
  • Fairley CK; Melbourne Sexual Health Centre, Alfred Health, Carlton, Victoria, Australia.
  • Howden BP; Central Clinical School, Monash University, Melbourne, Victoria, Australia.
  • Chen MY; The University of Queensland Centre for Clinical Research (UQ-CCR), Faculty of Medicine, The University of Queensland, Brisbane, Queensland, Australia.
  • Pasricha S; Melbourne Sexual Health Centre, Alfred Health, Carlton, Victoria, Australia.
  • Williamson DA; Central Clinical School, Monash University, Melbourne, Victoria, Australia.
J Clin Microbiol ; 59(5)2021 04 20.
Article in En | MEDLINE | ID: mdl-33658263
Resistance-guided therapy (RGT) for gonorrhea may reduce unnecessary use of broad-spectrum antibiotics. When reflexed from the Aptima Combo 2 assay, the ResistancePlus GC assay demonstrated 94.8% sensitivity and 100.0% specificity for Neisseria gonorrhoeae detection. Of the 379 concordant N. gonorrhoeae-positive samples, 86.8% were found to possess the gyrA S91F mutation, which was highly predictive for ciprofloxacin resistance and stable across 3,144 publicly available N. gonorrhoeae genomes. Our work supports the feasibility of implementing RGT for gonorrhea into routine molecular workflows.
Subject(s)
Key words
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Database: MEDLINE Main subject: Gonorrhea / Neisseria gonorrhoeae Type of study: Diagnostic_studies / Prognostic_studies Limits: Humans Language: En Year: 2021 Type: Article
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Database: MEDLINE Main subject: Gonorrhea / Neisseria gonorrhoeae Type of study: Diagnostic_studies / Prognostic_studies Limits: Humans Language: En Year: 2021 Type: Article