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New Gene Markers Expressed in Porcine Oviductal Epithelial Cells Cultured Primary In Vitro Are Involved in Ontological Groups Representing Physiological Processes of Porcine Oocytes.
Kulus, Magdalena; Kranc, Wieslawa; Wojtanowicz-Markiewicz, Katarzyna; Celichowski, Piotr; Swiatly-Blaszkiewicz, Agata; Matuszewska, Eliza; Sujka-Kordowska, Patrycja; Konwerska, Aneta; Zdun, Maciej; Bryl, Rut; Wieczorkiewicz, Maria; Kulus, Jakub; Stelmach, Boguslawa; Stefanska, Katarzyna; Budna-Tukan, Joanna; Petitte, James N; Mozdziak, Paul; Ratajczak, Kornel; Matysiak, Jan; Jaskowski, Jedrzej M; Nowicki, Michal; Kempisty, Bartosz.
Affiliation
  • Kulus M; Department of Veterinary Surgery, Institute of Veterinary Medicine, Nicolaus Copernicus University in Torun, 87-100 Torun, Poland.
  • Kranc W; Department of Anatomy, Poznan University of Medical Sciences, 60-781 Poznan, Poland.
  • Wojtanowicz-Markiewicz K; Department of Veterinary Surgery, Institute of Veterinary Medicine, Nicolaus Copernicus University in Torun, 87-100 Torun, Poland.
  • Celichowski P; Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznan, Poland.
  • Swiatly-Blaszkiewicz A; Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, 60-780 Poznan, Poland.
  • Matuszewska E; Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, 60-780 Poznan, Poland.
  • Sujka-Kordowska P; Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznan, Poland.
  • Konwerska A; Department of Anatomy and Histology, University of Zielona Gora, 65-046 Zielona Gora, Poland.
  • Zdun M; Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznan, Poland.
  • Bryl R; Department of Basic and Preclinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University in Torun, 87-100 Torun, Poland.
  • Wieczorkiewicz M; Department of Anatomy, Poznan University of Medical Sciences, 60-781 Poznan, Poland.
  • Kulus J; Department of Basic and Preclinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University in Torun, 87-100 Torun, Poland.
  • Stelmach B; Department of Diagnostics and Clinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University in Torun, 87-100 Torun, Poland.
  • Stefanska K; Division of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznan University of Medical Sciences, 60-535 Poznan, Poland.
  • Budna-Tukan J; Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznan, Poland.
  • Petitte JN; Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznan, Poland.
  • Mozdziak P; Prestage Department of Poultry Science, North Carolina State University, Raleigh, NC 27695, USA.
  • Ratajczak K; Prestage Department of Poultry Science, North Carolina State University, Raleigh, NC 27695, USA.
  • Matysiak J; Department of Veterinary Surgery, Institute of Veterinary Medicine, Nicolaus Copernicus University in Torun, 87-100 Torun, Poland.
  • Jaskowski JM; Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, 60-780 Poznan, Poland.
  • Nowicki M; Department of Diagnostics and Clinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University in Torun, 87-100 Torun, Poland.
  • Kempisty B; Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznan, Poland.
Int J Mol Sci ; 22(4)2021 Feb 19.
Article in En | MEDLINE | ID: mdl-33669854
Changes that occur within oviducts after fertilization are dependent on post-ovulation events, including oocyte-oviduct interactions. Although general processes are well-defined, the molecular basis are poorly understood. Recently, new marker genes involved in 'cell development', 'cell growth', 'cell differentiation' and 'cell maturation' processes have been identified in porcine oocytes. The aim of the study was to assess the expression profile of genes in primary in vitro cultured oviductal epithelial cells (OECs), clustered in Gene Ontology groups which enveloped markers also identified in porcine oocytes. OECs (from 45 gilts) were surgically removed and cultured in vitro for ≤ 30 days, and then subjected to molecular analyses. The transcriptomic and proteomic profiles of cells cultured during 7, 15 and 30 days were investigated. Additionally, morphological/histochemical analyzes were performed. The results of genes expression profiles were validated after using RT-qPCR. The results showed a significant upregulation of UNC45B, NOX4, VLDLR, ITGB3, FMOD, SGCE, COL1A2, LOX, LIPG, THY1 and downregulation of SERPINB2, CD274, TXNIP, CELA1, DDX60, CRABP2, SLC5A1, IDO1, ANPEP, FST. Detailed knowledge of the molecular pathways occurring in the OECs and the gametes that contact them may contribute both to developments of basic science of physiology, and new possibilities in advanced biotechnology of assisted reproduction.
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Full text: 1 Database: MEDLINE Main subject: Oocytes / Oviducts / Gene Expression Regulation / Epithelial Cells Limits: Animals Language: En Year: 2021 Type: Article

Full text: 1 Database: MEDLINE Main subject: Oocytes / Oviducts / Gene Expression Regulation / Epithelial Cells Limits: Animals Language: En Year: 2021 Type: Article