Assessing G4-Binding Ligands In Vitro and in Cellulo Using Dimeric Carbocyanine Dye Displacement Assay.

Desai, Nakshi; Shah, Viraj; Datta, Bhaskar

Desai, Nakshi; Shah, Viraj; Datta, Bhaskar.

Affiliation

Desai N; Department of Biological Engineering, Indian Institute of Technology, Gandhinagar 382355, India.
Shah V; Department of Biological Engineering, Indian Institute of Technology, Gandhinagar 382355, India.
Datta B; Department of Biological Engineering, Indian Institute of Technology, Gandhinagar 382355, India.

Molecules ; 26(5)2021 Mar 05.

Article in En | MEDLINE | ID: mdl-33807659

Subject(s)

Carbocyanines/chemistry; Carbocyanines/metabolism; G-Quadruplexes; Molecular Imaging/methods; DNA/chemistry; DNA/metabolism; Fluorescent Dyes/chemistry; HeLa Cells; Humans; Ligands; Nucleic Acid Conformation; Phthalazines/chemistry; Phthalazines/metabolism; Piperazines/chemistry; Piperazines/metabolism; Porphyrins/chemistry; Porphyrins/metabolism; RNA/chemistry; RNA/metabolism; Taq Polymerase/chemistry; Taq Polymerase/metabolism

Key words

DNA G-quadruplex; G4 ligands; G4 visualization; RNA G-quadruplex; fluorescence displacement assay; polymerase stop assay

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Full text: 1 Database: MEDLINE Main subject: Carbocyanines / G-Quadruplexes / Molecular Imaging Limits: Humans Language: En Year: 2021 Type: Article

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Full text: 1 Database: MEDLINE Main subject: Carbocyanines / G-Quadruplexes / Molecular Imaging Limits: Humans Language: En Year: 2021 Type: Article