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Protocol for derivation of organoids and patient-derived orthotopic xenografts from glioma patient tumors.
Oudin, Anaïs; Baus, Virginie; Barthelemy, Vanessa; Fabian, Carina; Klein, Eliane; Dieterle, Monika; Wantz, May; Hau, Ann-Christin; Dording, Claire; Bernard, Amandine; Michelucci, Alessandro; Yabo, Yahaya A; Kanli, Georgia; Keunen, Olivier; Bjerkvig, Rolf; Niclou, Simone P; Golebiewska, Anna.
Affiliation
  • Oudin A; NORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, Luxembourg.
  • Baus V; NORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, Luxembourg.
  • Barthelemy V; NORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, Luxembourg.
  • Fabian C; NORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, Luxembourg.
  • Klein E; Department of Biomedicine, University of Bergen, 5019 Bergen, Norway.
  • Dieterle M; NORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, Luxembourg.
  • Wantz M; NORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, Luxembourg.
  • Hau AC; NORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, Luxembourg.
  • Dording C; NORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, Luxembourg.
  • Bernard A; NORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, Luxembourg.
  • Michelucci A; NORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, Luxembourg.
  • Yabo YA; Neuro-Immunology Group, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, Luxembourg.
  • Kanli G; NORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, Luxembourg.
  • Keunen O; Neuro-Immunology Group, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, Luxembourg.
  • Bjerkvig R; NORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, Luxembourg.
  • Niclou SP; Faculty of Science, Technology and Medicine, University of Luxembourg, 4367 Belvaux, Luxembourg.
  • Golebiewska A; NORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, Luxembourg.
STAR Protoc ; 2(2): 100534, 2021 06 18.
Article in En | MEDLINE | ID: mdl-34027491
ABSTRACT
Tumor organoids and patient-derived orthotopic xenografts (PDOXs) are some of the most valuable pre-clinical tools in cancer research. In this protocol, we describe efficient derivation of organoids and PDOX models from glioma patient tumors. We provide detailed steps for organoid culture, intracranial implantation, and detection of tumors in the brain. We further present technical adjustments for standardized functional assays and drug testing. For complete details on the use and execution of this protocol, please refer to Golebiewska et al. (2020).
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Full text: 1 Database: MEDLINE Main subject: Brain Neoplasms / Drug Screening Assays, Antitumor / Organoids / Heterografts / Glioma Type of study: Guideline Limits: Animals / Female / Humans / Male Language: En Year: 2021 Type: Article
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Full text: 1 Database: MEDLINE Main subject: Brain Neoplasms / Drug Screening Assays, Antitumor / Organoids / Heterografts / Glioma Type of study: Guideline Limits: Animals / Female / Humans / Male Language: En Year: 2021 Type: Article