Direct Infusion Mass Spectrometry for Complex Lipid Analysis.
Methods Mol Biol
; 2295: 101-115, 2021.
Article
in En
| MEDLINE
| ID: mdl-34047974
ABSTRACT
Direct infusion or "shotgun" mass spectrometry provides a fast strategy to measure different classes of lipids, combining rapid analysis and short idle time. In contrast to liquid chromatography-mass spectrometry (LC-MS), the lipids are infused into the mass spectrometer without prior separation by liquid chromatography. Ions are separated in the quadrupole of a tandem mass spectrometer, and after collision-induced dissociation fragments are quantified relative to internal standards in the third quadrupole or in the time-of-flight mass analyzer of a triple quadrupole or quadrupole time of flight (Q-TOF) mass spectrometer. Abundant lipids, that is, galactolipids and phospholipids in leaves, are measured in crude lipid extracts, while less abundant lipids can be measured after enrichment by solid-phase extraction. Here we describe protocols for the quantification of the major plant glycerolipids (galactolipids, phospholipids, diacylglycerol, and triacylglycerol) using nanospray direct infusion mass spectrometry. This provides a strategy for comprehensive, highly sensitive, high-throughput lipidomic analyses.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Mass Spectrometry
/
Lipids
Language:
En
Year:
2021
Type:
Article