Enhancement of protein thermostability by three consecutive mutations using loop-walking method and machine learning.

Yoshida, Kazunori; Kawai, Shun; Fujitani, Masaya; Koikeda, Satoshi; Kato, Ryuji; Ema, Tadashi

Yoshida, Kazunori; Kawai, Shun; Fujitani, Masaya; Koikeda, Satoshi; Kato, Ryuji; Ema, Tadashi.

Affiliation

Yoshida K; Innovation Center, Amano Enzyme Inc., Technoplaza, Kakamigahara, Gifu, 509-0109, Japan.
Kawai S; Division of Applied Chemistry, Graduate School of Natural Science and Technology, Okayama University, Tsushima, Okayama, 700-8530, Japan.
Fujitani M; Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Nagoya, 464-8601, Japan.
Koikeda S; Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Nagoya, 464-8601, Japan.
Kato R; Innovation Center, Amano Enzyme Inc., Technoplaza, Kakamigahara, Gifu, 509-0109, Japan. satoshi_koikeda@amano-enzyme.com.
Ema T; Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Nagoya, 464-8601, Japan. kato-r@ps.nagoya-u.ac.jp.

Sci Rep ; 11(1): 11883, 2021 06 04.

Article in En | MEDLINE | ID: mdl-34088952

Subject(s)

Burkholderia cepacia/genetics; Enzyme Stability; Lipase/chemistry; Machine Learning; Mutagenesis; Mutation; Protein Engineering/methods; Proteins/chemistry; Proteins/genetics; Computational Biology; Escherichia coli/metabolism; Hot Temperature; Hydrolases/chemistry; Kinetics; Molecular Conformation; Molecular Dynamics Simulation; Mutagenesis, Site-Directed; Plasmids/metabolism; Polymerase Chain Reaction

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Full text: 1 Database: MEDLINE Main subject: Enzyme Stability / Protein Engineering / Proteins / Mutagenesis / Burkholderia cepacia / Machine Learning / Lipase / Mutation Type of study: Prognostic_studies Language: En Year: 2021 Type: Article

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