A novel global transcriptional perturbation target identified by forward genetics reprograms Vibrio natriegens for improving recombinant protein production.

Xu, Jiaqi; Yang, Junjie; Jiang, Yu; Wu, Mianbin; Yang, Sheng; Yang, Lirong

Xu, Jiaqi; Yang, Junjie; Jiang, Yu; Wu, Mianbin; Yang, Sheng; Yang, Lirong.

Affiliation

Xu J; Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310007, China.
Yang J; Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Jiang Y; Huzhou Center of Industrial Biotechnology, Shanghai Institutes for Biological Sciences, Huzhou 313000, China.
Wu M; Shanghai Taoyusheng Biotechnology Co. Ltd, Shanghai 201201, China.
Yang S; Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310007, China.
Yang L; Huzhou Center of Industrial Biotechnology, Shanghai Institutes for Biological Sciences, Huzhou 313000, China.

Acta Biochim Biophys Sin (Shanghai) ; 53(9): 1124-1133, 2021 Aug 31.

Article in En | MEDLINE | ID: mdl-34169308

Subject(s)

Recombinant Proteins/biosynthesis; Recombinant Proteins/genetics; Vibrio/enzymology; Vibrio/genetics; Biotechnology/methods; DNA-Directed RNA Polymerases/chemistry; DNA-Directed RNA Polymerases/genetics; Flow Cytometry; Green Fluorescent Proteins/biosynthesis; Green Fluorescent Proteins/genetics; High-Throughput Nucleotide Sequencing; High-Throughput Screening Assays; Molecular Biology/methods; Mutagenesis; Ochrobactrum anthropi/enzymology; Ochrobactrum anthropi/genetics; Plasmids; Transaminases/biosynthesis; Transaminases/genetics

Key words

Vibrio natriegens; forward genetics; global transcription reprogram; protein expression

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