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Quantifying the invasion and migration ability of cancer cells with a 3D Matrigel drop invasion assay.
Aslan, Merve; Hsu, En-Chi; Liu, Shiqin; Stoyanova, Tanya.
Affiliation
  • Aslan M; Department of Radiology, Canary Center at Stanford for Cancer Early Detection, Stanford University, 3155 Porter Drive. Palo Alto, CA 94304, USA.
  • Hsu EC; Department of Radiology, Canary Center at Stanford for Cancer Early Detection, Stanford University, 3155 Porter Drive. Palo Alto, CA 94304, USA.
  • Liu S; Department of Radiology, Canary Center at Stanford for Cancer Early Detection, Stanford University, 3155 Porter Drive. Palo Alto, CA 94304, USA.
  • Stoyanova T; Department of Radiology, Canary Center at Stanford for Cancer Early Detection, Stanford University, 3155 Porter Drive. Palo Alto, CA 94304, USA.
Biol Methods Protoc ; 6(1): bpab014, 2021.
Article in En | MEDLINE | ID: mdl-34377838
ABSTRACT
Metastasis is the main cause of cancer-associated morbidity which will account for ∼ 600,000 deaths in the USA in 2021. Defining new mechanisms that drive cancer metastasis is vital for developing new therapeutic strategies and improving clinical outcomes for cancer patients. Herein, we describe a recently established 3D Matrigel drop invasion assay to measure cancer cell invasion and migration capability in vitro. This assay is a versatile and simple tool to test the ability of cells to invade and migrate, test the functional role of genes of interest in cell invasion and migration, analyze the localization of the target proteins at the cell invasion edge in situ, and screen drug effects on cancer cell invasion and migration.
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Full text: 1 Database: MEDLINE Language: En Year: 2021 Type: Article
Fulltext
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Full text: 1 Database: MEDLINE Language: En Year: 2021 Type: Article