Effect of Vitamin C/Hydrocortisone Immobilization within Curdlan-Based Wound Dressings on In Vitro Cellular Response in Context of the Management of Chronic and Burn Wounds.
Int J Mol Sci
; 22(21)2021 Oct 25.
Article
in En
| MEDLINE
| ID: mdl-34768905
ABSTRACT
Bioactive dressings are usually produced using natural or synthetic polymers. Recently, special attention has been paid to ß-glucans that act as immunomodulators and have pro-healing properties. The aim of this research was to use ß-1,3-glucan (curdlan) as a base for the production of bioactive dressing materials (curdlan/agarose and curdlan/chitosan) that were additionally enriched with vitamin C and/or hydrocortisone to improve healing of chronic and burn wounds. The secondary goal of the study was to compressively evaluate biological properties of the biomaterials. In this work, it was shown that vitamin C/hydrocortisone-enriched biomaterials exhibited faster vitamin C release profile than hydrocortisone. Consecutive release of the drugs is a desired phenomenon since it protects wounds against accumulation of high and toxic concentrations of the bioactive molecules. Moreover, biomaterials showed gradual release of low doses of the hydrocortisone, which is beneficial during management of burn wounds with hypergranulation tissue. Among all tested variants of biomaterials, dressing materials enriched with hydrocortisone and a mixture of vitamin C/hydrocortisone showed the best therapeutic potential since they had the ability to significantly reduce MMP-2 synthesis by macrophages and increase TGF-ß1 release by skin cells. Moreover, materials containing hydrocortisone and its blend with vitamin C stimulated type I collagen deposition by fibroblasts and positively affected their migration and proliferation. Results of the experiments clearly showed that the developed biomaterials enriched with bioactive agents may be promising dressings for the management of non-healing chronic and burn wounds.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Ascorbic Acid
/
Wound Healing
/
Burns
/
Hydrocortisone
/
Keratinocytes
/
Beta-Glucans
/
Fibroblasts
Type of study:
Etiology_studies
Limits:
Humans
Language:
En
Year:
2021
Type:
Article