Agrobacterium leguminum sp. nov., isolated from nodules of Phaseolus vulgaris in Spain.
Int J Syst Evol Microbiol
; 71(12)2021 Dec.
Article
in En
| MEDLINE
| ID: mdl-34870578
ABSTRACT
Two endophytic strains, coded MOVP5T and MOPV6, were isolated from nodules of Phaseolus vulgaris plants grown on agricultural soil in Southeastern Spain, and were characterized through a polyphasic taxonomy approach. Their 16S rRNA gene sequences showed 99.3 and 99.4â%, 98.9 and 99.6â%, and 99.0 and 98.7% similarity to 'A. deltaense' YIC 4121T, A. radiobacter LGM 140T, and A. pusense NRCPB10T, respectively. Multilocus sequence analysis based on sequences of recA and atpD genes suggested that these two strains could represent a new Agrobacterium species with less than 96.5â% similarity to their closest relatives. PCR amplification of the telA gene, involved in synthesis of protelomerase, confirmed the affiliation of strains MOPV5T and MOPV6 to the genus Agrobacterium. Whole genome average nucleotide identity and digital DNA-DNA hybridization average values were less than 95.1 and 66.7â%, respectively, with respect to its closest related species. Major fatty acids in strain MOPV5T were C18ââ1 ω7c/C18ââ1 ω6c in summed feature 8, C19ââ0 cyclo ω8c, C16ââ0 and C16ââ0 3-OH. Colonies were small to medium, pearl-white coloured on YMA at 28 °C and growth was observed at 10-42 °C, pH 5.0-10.0 and with 0.0-0.5â% (w/v) NaCl. The DNA G+C content was 59.9 mol%. These two strains differ from all other genomovars of Agrobacterium found so far, including those that have not yet given a Latin name. The combined genotypic, phenotypic and chemotaxonomic data support the classification of strain MOPV5T as representing a novel species of Agrobacterium, for which the name Agrobacterium leguminum sp. nov. is proposed. The type strain is MOPV5T (=CECT 30096T=LMG 31779T).
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Phylogeny
/
Phaseolus
/
Root Nodules, Plant
/
Agrobacterium
Country/Region as subject:
Europa
Language:
En
Year:
2021
Type:
Article