Cryo-EM structures of human RNA polymerase I.
Nat Struct Mol Biol
; 28(12): 997-1008, 2021 12.
Article
in En
| MEDLINE
| ID: mdl-34887565
ABSTRACT
RNA polymerase I (Pol I) specifically synthesizes ribosomal RNA. Pol I upregulation is linked to cancer, while mutations in the Pol I machinery lead to developmental disorders. Here we report the cryo-EM structure of elongating human Pol I at 2.7 Å resolution. In the exit tunnel, we observe a double-stranded RNA helix that may support Pol I processivity. Our structure confirms that human Pol I consists of 13 subunits with only one subunit forming the Pol I stalk. Additionally, the structure of human Pol I in complex with the initiation factor RRN3 at 3.1 Å resolution reveals stalk flipping upon RRN3 binding. We also observe an inactivated state of human Pol I bound to an open DNA scaffold at 3.3 Å resolution. Lastly, the high-resolution structure of human Pol I allows mapping of disease-related mutations that can aid understanding of disease etiology.
Full text:
1
Database:
MEDLINE
Main subject:
RNA Polymerase I
/
Pol1 Transcription Initiation Complex Proteins
/
Neoplasms
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Year:
2021
Type:
Article