Detection and enumeration of Lak megaphages in microbiome samples by endpoint and quantitative PCR.
STAR Protoc
; 3(1): 101029, 2022 03 18.
Article
in En
| MEDLINE
| ID: mdl-35059650
ABSTRACT
Lak megaphages are prevalent across diverse gut microbiomes and may potentially impact animal and human health through lysis of Prevotella. Given their large genome size (up to 660 kbp), Lak megaphages are difficult to culture, and their identification relies on molecular techniques. Here, we present optimized protocols for identifying Lak phages in various microbiome samples, including procedures for DNA extraction, followed by detection and quantification of genes encoding Lak structural proteins using diagnostic endpoint and SYBR green-based quantitative PCR, respectively. For complete details on the use and execution of this protocol, please refer to Crisci et al., (2021).
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Bacteriophages
/
Microbiota
/
Gastrointestinal Microbiome
Type of study:
Diagnostic_studies
/
Guideline
Limits:
Animals
Language:
En
Year:
2022
Type:
Article