Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm.
Int J Mol Sci
; 23(3)2022 Jan 31.
Article
in En
| MEDLINE
| ID: mdl-35163562
ABSTRACT
Optical microscopy has vastly expanded the frontiers of structural and functional biology, due to the non-invasive probing of dynamic volumes in vivo. However, traditional widefield microscopy illuminating the entire field of view (FOV) is adversely affected by out-of-focus light scatter. Consequently, standard upright or inverted microscopes are inept in sampling diffraction-limited volumes smaller than the optical system's point spread function (PSF). Over the last few decades, several planar and structured (sinusoidal) illumination modalities have offered unprecedented access to sub-cellular organelles and 4D (3D + time) image acquisition. Furthermore, these optical sectioning systems remain unaffected by the size of biological samples, providing high signal-to-noise (SNR) ratios for objective lenses (OLs) with long working distances (WDs). This review aims to guide biologists regarding planar illumination strategies, capable of harnessing sub-micron spatial resolution with a millimeter depth of penetration.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Imaging, Three-Dimensional
/
Time-Lapse Imaging
/
Single Molecule Imaging
Language:
En
Year:
2022
Type:
Article