Decoding Acinetobacter baumannii biofilm dynamics and associated protein markers: proteomic and bioinformatics approach.
Arch Microbiol
; 204(4): 200, 2022 Mar 03.
Article
in En
| MEDLINE
| ID: mdl-35239017
ABSTRACT
Biofilm formation by Acinetobacter baumannii is one of the major cause of its persistence in hospital environment. Biofilm phenotypes are more resistant to physical as well as chemical stresses than their planktonic counterparts. The present study was carried in quest of biofilm-associated protein markers and their association with various biological pathways of A. baumannii. The study was designed with an aim to highlight the crucial common factor present in the majority of the A. baumannii strains irrespective of its resistance nature. A label-free proteome comparison of biofilm and planktonic phenotypes of A. baumannii was done using QExactive tandem mass spectrometry. Our investigation suggests key elevation of adhesion factors, acetate metabolism, nutrient transporters, and secretion system proteins are required for biofilm formation in A. baumannii. Elevation of biofilm-associated proteins revealed that biofilm is the unique phenotype with the potential to form robust matrix-embedded colonies and defeat stress condition. Further, core protein markers of biofilm phenotypes could be used as targets for new clinical interventions to combat biofilm-associated infections.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Acinetobacter baumannii
Type of study:
Risk_factors_studies
Language:
En
Year:
2022
Type:
Article