Dynamics of Drosophila endoderm specification.
Proc Natl Acad Sci U S A
; 119(15): e2112892119, 2022 04 12.
Article
in En
| MEDLINE
| ID: mdl-35412853
ABSTRACT
During early Drosophila embryogenesis, a network of gene regulatory interactions orchestrates terminal patterning, playing a critical role in the subsequent formation of the gut. We utilized CRISPR gene editing at endogenous loci to create live reporters of transcription and light-sheet microscopy to monitor the individual components of the posterior gut patterning network across 90 min prior to gastrulation. We developed a computational approach for fusing imaging datasets of the individual components into a common multivariable trajectory. Data fusion revealed low intrinsic dimensionality of posterior patterning and cell fate specification in wild-type embryos. The simple structure that we uncovered allowed us to construct a model of interactions within the posterior patterning regulatory network and make testable predictions about its dynamics at the protein level. The presented data fusion strategy is a step toward establishing a unified framework that would explore how stochastic spatiotemporal signals give rise to highly reproducible morphogenetic outcomes.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Body Patterning
/
Drosophila Proteins
/
Drosophila melanogaster
/
Endoderm
/
Gene Regulatory Networks
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Year:
2022
Type:
Article