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Generation of Calpain-3 knock-out porcine embryos by CRISPR-Cas9 electroporation and intracytoplasmic microinjection of oocytes before insemination.
Navarro-Serna, Sergio; Dehesa-Etxebeste, Martxel; Piñeiro-Silva, Celia; Romar, Raquel; Lopes, Jordana S; López de Munaín, Adolfo; Gadea, Joaquín.
Affiliation
  • Navarro-Serna S; University of Murcia Dept. Physiology, Murcia, Spain; International Excellence Campus for Higher Education and Research "Campus Mare Nostrum" and Institute for Biomedical Research of Murcia (IMIB-Arrixaca), Murcia, Spain.
  • Dehesa-Etxebeste M; IIS Biodonostia, Neuroscience, San Sebastián, Spain.
  • Piñeiro-Silva C; University of Murcia Dept. Physiology, Murcia, Spain; International Excellence Campus for Higher Education and Research "Campus Mare Nostrum" and Institute for Biomedical Research of Murcia (IMIB-Arrixaca), Murcia, Spain.
  • Romar R; University of Murcia Dept. Physiology, Murcia, Spain; International Excellence Campus for Higher Education and Research "Campus Mare Nostrum" and Institute for Biomedical Research of Murcia (IMIB-Arrixaca), Murcia, Spain.
  • Lopes JS; University of Murcia Dept. Physiology, Murcia, Spain; International Excellence Campus for Higher Education and Research "Campus Mare Nostrum" and Institute for Biomedical Research of Murcia (IMIB-Arrixaca), Murcia, Spain.
  • López de Munaín A; IIS Biodonostia, Neuroscience, San Sebastián, Spain; Department of Neurology, Hospital Universitario Donostia-OSAKIDETZA, San Sebastián, Spain; Department of Neurosciences, University of the Basque Country (UPV-EHU), San Sebastián, Spain; CIBERNED, CIBER, Institute Carlos III, Madrid, Spain.
  • Gadea J; University of Murcia Dept. Physiology, Murcia, Spain; International Excellence Campus for Higher Education and Research "Campus Mare Nostrum" and Institute for Biomedical Research of Murcia (IMIB-Arrixaca), Murcia, Spain. Electronic address: jgadea@um.es.
Theriogenology ; 186: 175-184, 2022 Jul 01.
Article in En | MEDLINE | ID: mdl-35500431
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Full text: 1 Database: MEDLINE Main subject: Calpain / CRISPR-Cas Systems Limits: Animals Language: En Year: 2022 Type: Article
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PubMed Links
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Full text: 1 Database: MEDLINE Main subject: Calpain / CRISPR-Cas Systems Limits: Animals Language: En Year: 2022 Type: Article