Hot isopropanol quenching procedure for automated microtiter plate scale <sup>13</sup>C-labeling experiments.

Nießer, Jochen; Müller, Moritz Fabian; Kappelmann, Jannick; Wiechert, Wolfgang; Noack, Stephan

Hot isopropanol quenching procedure for automated microtiter plate scale ¹³C-labeling experiments.

Nießer, Jochen; Müller, Moritz Fabian; Kappelmann, Jannick; Wiechert, Wolfgang; Noack, Stephan.

Affiliation

Nießer J; Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, 52425, Jülich, Germany.
Müller MF; Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, 52425, Jülich, Germany.
Kappelmann J; Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, 52425, Jülich, Germany.
Wiechert W; Currenta, GmbH & Co. OHG, 51368, Leverkusen, Germany.
Noack S; Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, 52425, Jülich, Germany.

Microb Cell Fact ; 21(1): 78, 2022 May 09.

Article in En | MEDLINE | ID: mdl-35527247

Subject(s)

2-Propanol; Corynebacterium glutamicum; 2-Propanol/metabolism; Amino Acids/metabolism; Carbon Isotopes/metabolism; Corynebacterium glutamicum/metabolism; Isotope Labeling/methods

Key words

13C-labeling; Boiling solvent quenching; Corynebacterium glutamicum; Isotopic labeling; Isotopically transient experiment; Lab automation; Metabolic quenching; Microbioreactor cultivation

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