Quantitation of non-derivatized free amino acids for detecting inborn errors of metabolism by incorporating mixed-mode chromatography with tandem mass spectrometry.
J Mass Spectrom Adv Clin Lab
; 25: 1-11, 2022 Aug.
Article
in En
| MEDLINE
| ID: mdl-35637738
AMR, analytical measurement range; AQC, 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate; ASA, argininosuccinic acid; Allo-isoleucine; Amino acid; CEX, cation exchange; CS, Fcerebrospinal fluid; CV, coefficient of variation; GABA, gamma-aminobutyric acid; GC/MS, gas chromatography-mass spectrometry; HCl, hydrochloric acid; HILIC, hydrophilic interaction liquid chromatography; IS, internal standard; Inborn errors of metabolism; LC-MS/MS; LC-MS/MS, liquid chromatography-tandem mass spectrometry; LLOQ, lower limit of quantitation; MM, mixed-mode; MS/MS, tandem mass spectrometry; MSU, Dmaple syrup urine disease; Maple syrup urine disease; QC, quality control; RPL, Creversed phase liquid chromatography; ULO, Qupper limit of quantitation