The new X-ray/visible microscopy MAXWELL technique for fast three-dimensional nanoimaging with isotropic resolution.

Kohmura, Yoshiki; Yang, Shun-Min; Chen, Hsiang-Hsin; Takano, Hidekazu; Chang, Chia-Ju; Wang, Ya-Sian; Lee, Tsung-Tse; Chiu, Ching-Yu; Yang, Kai-En; Chien, Yu-Ting; Hu, Huan-Ming; Su, Tzu-Ling; Petibois, Cyril; Chen, Yi-Yun; Hsu, Cheng-Huan; Chen, Peilin; Hueng, Dueng-Yuan; Chen, Shean-Jen; Yang, Chi Lin; Chin, An-Lun; Low, Chian-Ming; Tan, Francis Chee Kuan; Teo, Alvin; Tok, Eng Soon; Cai, Xu Xiang; Lin, Hong-Ming; Boeckl, John; Stampfl, Anton P; Yamada, Jumpei; Matsuyama, Satoshi; Ishikawa, Tetsuya; Margaritondo, Giorgio; Chiang, Ann-Shyn; Hwu, Yeukuang

Kohmura, Yoshiki; Yang, Shun-Min; Chen, Hsiang-Hsin; Takano, Hidekazu; Chang, Chia-Ju; Wang, Ya-Sian; Lee, Tsung-Tse; Chiu, Ching-Yu; Yang, Kai-En; Chien, Yu-Ting; Hu, Huan-Ming; Su, Tzu-Ling; Petibois, Cyril; Chen, Yi-Yun; Hsu, Cheng-Huan; Chen, Peilin; Hueng, Dueng-Yuan; Chen, Shean-Jen; Yang, Chi Lin; Chin, An-Lun; Low, Chian-Ming; Tan, Francis Chee Kuan; Teo, Alvin; Tok, Eng Soon; Cai, Xu Xiang; Lin, Hong-Ming; Boeckl, John; Stampfl, Anton P; Yamada, Jumpei; Matsuyama, Satoshi; Ishikawa, Tetsuya; Margaritondo, Giorgio; Chiang, Ann-Shyn; Hwu, Yeukuang.

Affiliation

Kohmura Y; RIKEN/SPring-8 Center, Hyogo, 679-5148, Japan. yoshiki.kohmura@riken.jp.
Yang SM; Institute of Physics, Academia Sinica, Nankang, Taipei, 11529, Taiwan.
Chen HH; Institute of Physics, Academia Sinica, Nankang, Taipei, 11529, Taiwan.
Takano H; RIKEN/SPring-8 Center, Hyogo, 679-5148, Japan.
Chang CJ; Institute of Physics, Academia Sinica, Nankang, Taipei, 11529, Taiwan.
Wang YS; Institute of Physics, Academia Sinica, Nankang, Taipei, 11529, Taiwan.
Lee TT; Institute of Physics, Academia Sinica, Nankang, Taipei, 11529, Taiwan.
Chiu CY; Institute of Physics, Academia Sinica, Nankang, Taipei, 11529, Taiwan.
Yang KE; Institute of Physics, Academia Sinica, Nankang, Taipei, 11529, Taiwan.
Chien YT; Institute of Physics, Academia Sinica, Nankang, Taipei, 11529, Taiwan.
Hu HM; Institute of Physics, Academia Sinica, Nankang, Taipei, 11529, Taiwan.
Su TL; Institute of Physics, Academia Sinica, Nankang, Taipei, 11529, Taiwan.
Petibois C; Institute of Physics, Academia Sinica, Nankang, Taipei, 11529, Taiwan.
Chen YY; Institute of Physics, Academia Sinica, Nankang, Taipei, 11529, Taiwan.
Hsu CH; Institute of Physics, Academia Sinica, Nankang, Taipei, 11529, Taiwan.
Chen P; Institute of Physics, Academia Sinica, Nankang, Taipei, 11529, Taiwan.
Hueng DY; Research Center for Applied Sciences, Academia Sinica, Taipei, 11529, Taiwan.
Chen SJ; Department of Surgery, School of Medicine, National Defense Medical Center, Taipei, Taiwan.
Yang CL; College of Photonics, National Yang Ming Chiao Tung University, Tainan, Taiwan.
Chin AL; Brain Research Center, National Tsing Hua University, Hsinchu, Taiwan.
Low CM; Brain Research Center, National Tsing Hua University, Hsinchu, Taiwan.
Tan FCK; Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.
Teo A; Department of Anaesthesia, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.
Tok ES; Department of Anaesthesia, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.
Cai XX; School of Chemical and Life Sciences, Nanyang Polytechnic, Singapore, Singapore.
Lin HM; ÆMaGIC-Lab, Department of Physics, National University of Singapore, Singapore, Singapore.
Boeckl J; Mechanical and Materials Department, Tatung University, Taipei, Taiwan.
Stampfl AP; Mechanical and Materials Department, Tatung University, Taipei, Taiwan.
Yamada J; US Air Force Research Laboratory, Materials and Manufacturing Directorate, WPAFB, Fairborn, OH, 43455, USA.
Matsuyama S; Australian Nuclear Science and Technology Organisation, Sydney, NSW, 2234, Australia.
Ishikawa T; RIKEN/SPring-8 Center, Hyogo, 679-5148, Japan.
Margaritondo G; Department of Materials Physics, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa, Nagoya, 464-8603, Japan.
Chiang AS; RIKEN/SPring-8 Center, Hyogo, 679-5148, Japan.
Hwu Y; Ecole Polytechnique Fédérale de Lausanne, 1015, Lausanne, Switzerland.

Sci Rep ; 12(1): 9668, 2022 06 11.

Article in En | MEDLINE | ID: mdl-35690597

ABSTRACT

ABSTRACT

Microscopy by Achromatic X-rays With Emission of Laminar Light (MAXWELL) is a new X-ray/visible technique with attractive characteristics including isotropic resolution in all directions, large-volume imaging and high throughput. An ultrathin, laminar X-ray beam produced by a Wolter type I mirror irradiates the sample stimulating the emission of visible light by scintillating nanoparticles, captured by an optical system. Three-dimensional (3D) images are obtained by scanning the specimen with respect to the laminar beam. We implemented and tested the technique with a high-brightness undulator at SPring-8, demonstrating its validity for a variety of specimens. This work was performed under the Synchrotrons for Neuroscience-an Asia-Pacific Strategic Enterprise (SYNAPSE) collaboration.

Subject(s)

Microscopy; Synchrotrons; Imaging, Three-Dimensional; Light; Microscopy/methods; Tomography, X-Ray Computed/methods; X-Rays

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